The aim of Photobiomodulation to accelerate tissue healing, reduce inflammation and relief the pain; regarding these points we will discuss the clinical targets and trigger points. Also we will show the clinical application step by step as the diagnosis, protocol ,setting and safety. The effective dose and the amount of energy to get the best dose for the treatment. We will assess different handpieces that used in the treatment . New Application to calculate Parameters for all types of lasers for all medical and dental fields in a professional way as it depends on distance of the tissue to the tip , Gaussian and top hat profile, also there is a specific way to calculate parameters of photobiomodulation.